Anti-His-tag pAb

Product Code:

MBL-PM032

Product Group:

Primary Antibodies

Supplier:

MBL

Host Type:

Rabbit

Antibody Isotype:

IgG

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Applications:

Immunoprecipitation (IP)
Western Blot (WB)

Shipping:

4°C

Storage:

-20°C

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Code	Size	Price

MBL-PM032

100 ul

£296.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Applications:

WB - 1:1000 IP - 5 uL/sample

Background:

Expression vectors containing a protein and a tag peptide are commonly used. His-Tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-Tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.

Formulation:

In PBS/50% glycerol, pH 7.2

Gene IDs:

Human: 57016 Mouse: 15109

Immunogen Translated:

6xHis synthetic peptide carrier protein binding

Reactivity:

This antibody recognizes His-tagged protein on Western blotting and Immunoprecipitation.

Shelf Life:

1 year

Source:

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated 6xHis synthetic peptide.

Target:

His

Documents

Datasheet

References

1) Nishiyama, A., et al., Nature 502, 249-253 (2013) [WB] 2) Takemoto, M., et al.,Cereb.Cortex 21, 1925-1934 (2011) [IC] 3) Kojima, K., et al., Genes Cells 14, 1155-1165 (2009) [IP] 4) Lee, J. H., et al., J. Virol. 80, 3844-3852 (2006) 5) Muro, S., et al., J. Cell Sci. 116, 1599-1609 (2003) 6) Isoyama, T., et al., J. Biol. Chem. 277, 39634-39641 (2002) 7) Isoyama, T., et al., J. Biol. Chem. 276, 21863-21869 (2001) 8) Toshima, J., et al., Mol. Biol. Cell 12, 1131-1145 (2001)