Anti-T7-tag pAb

WB - 1:1,000 (chemiluminescence detection system) IP - 5 uL/sample

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The T7 Tag Antibody is directed against the 11 amino acid gene 10 leader peptide expressed by many translation vectors (MASMTGGQQMG). Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage. Anti-T7-Tag antibodies are useful reagents to easily identify, detect, or purify T7-tag fusion proteins from cell lysates.

100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Binding synthetic peptide carrier protein (MASMTGGQQMG)

This antibody reacts with T7-Tag on Western blotting.

1 year

This antibody was purified from rabbit serum using affinity chromatography. The rabbit was immunized with thyroglobulin (TG) conjugated synthetic peptide, TG-MASMTGGQQMGC.

T7