Anti-S-tag pAb

WB - 1:1,000 (chemiluminescence detection system) IP - 5 uL/sample

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-Tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-Tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-Tag fusion proteins by enzymatic assays. However, antibody against the S-Tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-Tag containing fusion proteins.

100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.

Binding synthetic peptide carrier protein (CP-KETAAAKFERQHIDSC)

This antibody reacts with S-tagged protein on Western blotting and Immunoprecipitation.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP -KETAAAKFERQHIDS.

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