MBL

Anti-DDDDK-tag pAb-Agarose

Product Code:
 
MBL-PM020-8
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Immunoprecipitation (IP)
Shipping:
 
4°C
Storage:
 
4°C
1 / 1
❮❯

No additional charges, what you see is what you pay! *

CodeSizePrice
MBL-PM020-8Gel: 200 uL£307.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Show All

Further Information

Applications:
IP - 20 uL
Background:
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein?s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (DYKDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibody is optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins.
Conjugate:
Agarose
Formulation:
320 ug of anti-DDDDK-tagpolyclonal antibody covalently coupled to 200 ul ofagarose gel and provided as a 50% gel slurry suspended inPBS containing preservative (0.09% sodium azide) for atotal volume of 400 ul.
Immunogen Translated:
Synthetic peptide binding KLH, DYKDDDDK
Reactivity:
This antibody recognizes DDDDK-tag peptide sequence (DYKDDDDK) on Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with KLH conjugated DYKDDDDK peptide
Target:
DDDDK

Documents

References

1) Tan, L. J., et al., Genes Cells 17, 173-185 (2012) 2) Fukunaka, A., et al., J. Biol. Chem. 286, 16363-16373 (2011) 3) Takahashi , S., et al., J. Cell Sci. 122, 985-994 (2009) 4) Maehara, T., et al., J. Biol. Chem. 283, 35053-35059 (2008) This antibody is used in these references.