MBL

Anti-α-Tubulin mAb

Product Code:
 
MBL-M175-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2F9
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Hamster
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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CodeSizePrice
MBL-M175-3100 ul£269.00
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  • Further Information
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Further Information

Applications:
WB - 2 ug/mL (chemiluminescence detection system) IP - 5 ug/200 uL of cell extract from 2x106 cells ICC - 2 ug/mL
Background:
Microtubules are one of the components of the cytoskeleton, which performs essential and diverse functions within eukaryotic cells. Microtubules are composed of a heterodimer of α and β tubulins. Tubulin is a GTP-binding protein, and extension and shortening of the microtubules are regulated by binding/hydrolysis of GTP.
Concentration:
2 mg/mL
Formulation:
200 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 7846 Mouse: 22142 Rat: 64158
Immunogen Translated:
α-Tubulin N-terminal synthetic peptide of human
Reactivity:
This antibody reacts with human α-tubulin on western blotting, Immunoprecipitation, and Immunocytochemistry. The reactivity to mouse, rat, hamster and chicken α-tubulin was confirmed by Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 2F9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the synthetic peptide corresponding to N-terminal of human α-Tubulin.

Documents

References

1) Heald, R., and Nogales, E., J. Cell Sci. 115, 3-4 (2002) 2) Hall, J. L., and Cowan, N. J., Nucleic Acids Res. 13, 207-223 (1985)