MBL

Anti-GAPDH mAb

Product Code:
 
MBL-M171-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3H12
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Hamster
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
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MBL-M171-3100 ul£301.00
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  • Further Information
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Further Information

Applications:
WB - 3 ug/mL (chemiluminescence detection system)
Background:
GAPDH (Glyceraldehyde- 3-phosphate Dehydrogenase) is a well-known enzyme, which catalyzes of glycolysis. GAPDH is stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping protein. Therefore, GAPDH is often used as a loading control of WB.
Concentration:
3 mg/mL
Formulation:
300 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 2597 Mouse: 14433
Immunogen Translated:
GAPDH from rabbit muscle
Reactivity:
This antibody reacts with GAPDH on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 3H12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with CH3 mouse lymphocyte immunized with the GAPDH from rabbit muscle.
Target:
GAPDH

Documents

References

1) Tarze, A., et al., Oncogene 26, 2606-2620 (2007) 2) Barber, R. D., et al., Physiol. Genomics. 21, 389-395 (2005)