Anti-monomeric Kusabira-Green C-terminal fragment mAb

WB - 1 ug/mL (chemiluminescence detection system)

CoralHue® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue®mKG_N fragment and CoralHue®mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don?t interact, CoralHue®mKG_N fragment and CoralHue®mKG_C fragment can not approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue®mKG fragments spatially approach each other and the local effective concentration increases. As a result, CoralHue®mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions. Clone 21B10 has the epitope on CoralHue®mKG_C fragment and can detect the fusion protein with CoralHue®mKG_C specifically.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

mKG_C (1-51 a.a.)

This antibody reacts with CoralHue® mKG_C fragment and CoralHue® mKO2 on Western blotting.

1 year

This antibody was purified from hybridoma (clone 21B10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the CoralHue® mKG_C protein (51 aa).

Kusabira-Green