Anti-Caspase-3 (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system)

Caspase-3 (also known as CPP32, Yama, apopain) is a key member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, -8, or -9. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form the active tetrameric enzyme tetramer (a pair of heterodimers). The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP, SREBPs, and DFF). Activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Human: 836 Mouse: 12367

(Total length) Recombinant human Caspase-3

This antibody reacts with Pro-Caspase-3 (32kDa) and active Caspase-3 (17kDa) on Western blotting.

1 year

This antibody was purified from hybridoma (clone 1F3) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Wister rat splenocyte immunized with full length human recombinant Caspase-3.

Caspase-3