MBL

Anti-Luciferase mAb

Product Code:
 
MBL-M095-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2D4
Regulatory Status:
 
RUO
Target Species:
 
Plant
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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CodeSizePrice
MBL-M095-3100 ug£280.00
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  • Further Information
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Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 5 ug / 200 uL of cell extract from 5x106 cells
Background:
Luciferase is a 61 kDa luminescent enzyme isolated from Photinus pyralis (North American Firefly). The enzyme catalyzes the ATP dependent oxidation of beetle luciferin to produce inorganic phosphate and light. Luciferase is frequently used as a reporter protein to quantify expression levels from promoters in tissue culture and in vivo.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Immunogen Translated:
Length recombinant luciferase (photinus pyralis)
Reactivity:
This antibody reacts with Luciferase (61kDa) on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 2D4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-U1 with Balb/c mouse lymphocyte immunized with recombinant full length luciferase (photinus pyralis).
Target:
Luciferase

Documents

References

1) Xu, G., et al., Mol. Pharmacol. 59, 485-492 (2001)