Anti-Phospho-RB (Ser780) (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system)

Cyclin-dependent kinases (Cdks) play important roles in the regulation of the cell cycle. RB protein (pRB) is phosphorylated by cyclin D-Cdk4/Cdk6 and cyclin A/cyclin E-Cdk2 during the G1/S transition. This phosphorylation causes the inactivation of the growth inhibitory functions of pRB. pRB undergo phosphorylationm and attendant functional inactivation, the cell proceed into late G1. Cyclin D-Cdk4 specifically phosphorylates Ser780 in pRB, while cyclin E-Cdk2 and cyclin A-Cdk2 does not.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 5925 Mouse: 19645

synthetic human phospho-RB (Ser780) peptide, TRPPTLS (PO3) PIPHIP, which corresponding to amino acids 774-786 of human pR

This antibody recognizes only phosphorylation - site sequence, RPPTLS(PO3)-PIPHIPR. This antibody detects the 115 kDa human phospho-RB protein by Western blotting.

1 year

This antibody was purified from hybridoma (clone 2C4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with synthetic human phospho-RB (Ser780) peptide, TRPPTLS (PO3) PIPHIP, which corresponding to amino acids 774-786 of human pRB.

RB