Anti-Caspase-8 (Human) mAb
Product Code:
MBL-M032-3
MBL-M032-3
Host Type:
Mouse
Mouse
Antibody Isotype:
IgG2b
IgG2b
Antibody Clonality:
Monoclonal
Monoclonal
Antibody Clone:
5F7
5F7
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Application:
Western Blot (WB)
Western Blot (WB)
Shipping:
4°C
4°C
Storage:
-20°C
-20°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| MBL-M032-3 | 100 ug | £296.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Typical lead time: 10-14 working days.
Contact us for more accurate information.
- Further Information
- Documents
- References
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Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system)
Background:
Caspase-8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin-1 converting enzyme)/CED-3 family cysteine protease. It is the most upstream protease that receives the activation signal from the Fas (APO1/CD95) and TNFR1 (Tumor Necrosis Factor Receptor 1) to initiate the apoptotic protease cascade that leads to activation of ICE/CED-3 family proteases. Caspase-8 has high homologous region to the ICE/CED-3 family in C-terminal and two death effecter domains (DED) in N-terminal. Binding of caspase-8 to FADD (MORT1) through association of their DED, and consequent activation of the caspases by their proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR1-induced apoptosis. Recently the inhibitor of Fasand TNFR1-induced apoptosis is identified, called I-FLICE (FLIP/Casper/FLAME/CASH). I-FLICE has high homology to caspase-8 and it contains two DED, which interacts with caspase-8 and FADD, and potently inhibits Fas- and TNFR1-induced apoptosis.
Formulation:
1.0 mg/mL in PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 22861 Mouse: 12370
Immunogen Translated:
Recombinant human Caspase-8 (:176-460 aa terminal C)
Reactivity:
This antibody reacts with human
caspase-8 on Western blotting. It doesn't cross-react with
other caspases including caspase-10 (FLICE2) which has
high homology to caspase-8, however cross-reactivity with
I-FLICE has not been examined.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein A agarose. This hybridoma was
established by fusion of mouse myeloma P3U1 with Balb/c
mouse splenocyte immunized with the recombinant human
FLICE corresponding to C-terminal amino acids (176-460
aa).
Target:
Caspase-8
Documents
References
Stagni, V., et al., Blood 111, 829-837 (2008)
Okamoto, K., et al., Genes Cells 11, 177-191 (2006)
Aaron D. Schimmer., et al., Cancer Res. 66, 2367-2375 (2006) Ohgushi, M., et al., Mol. Cell Biol. 25, 10017-10028 (2005)
Imamura, R., et al., J. Biol. Chem. 279, 46415-46423 (2004)
Nakayama, M., et al., J. Immunol. 168, 734-743 (2002)
Imai, Y., et al., Nature 398, 777-785 (1999)
Eguchi, Y., et al., Cancer Res. 59, 2174-2181 (1999)
Kawahara, A., et al., Oncogene 17, 2549-2554 (1998)
Muzio, M. et al., J. Biol. Chem. 272, 2952-2956 (1997)
Hu, S. et al., J. Biol. Chem. 272, 17255-17257 (1997)
Irmler, M. et al., Nature 388, 190-195 (1997)
Scaffidi, C. et al., J. Biol. Chem. 272, 26953-26958 (1997)
Boldin, M. P. et al., Cell 85, 803-815 (1996)
Muzio, M. et al., Cell 85, 817-827 (1996)