Anti-Phospho-DNA Topoisomerase IIα (Thr1342) (Human) mAb

WB - 1-10 ug/mL (chemiluminescence detection system) ICC - 10 ug/mL

Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo IIα (170 kDa) and Topo IIβ (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo IIα is expressed constantly throughout cell cycle, whereas the expression of Topo IIβ is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. It is considered that Topo IIα plays an essential role in cell proliferation, especially during late S to M phase. Threonine 1342 in human Topo IIα is phosphorylated throughout the cell cycle. Phosphorylation level of threonine 1342 in G2 to M phase is as twice as much in G1 or S phase. Anti-phospho-Topoisomerase IIα antibody recognize phosphorylated threonine residue, PT 1342 in human Topo II? specifically and does not react with non-phosphorylated form.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 7150 Mouse: 21969

Topo IIα oxidation Humanrin synthetic peptide (Thr1342) (FSDFDEK (p) TDDEDFVPC 1335-1349 aa)

This antibody reacts with human phospho-Topo IIα (170 kDa) on Western blotting.

1 year

This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone 3D4) was established by fusion of mouse myeloma cell SP2/0-Ag14 with Balb/c mouse splenocyte immunized with the human Topo II? synthetic phosphopeptide corresponding to FSDFDEK(p)TDDEDFVPC (1335-1349 aa)