MBL

Anti-MFG-E8 (Mouse) mAb

Product Code:
 
MBL-D161-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Hamster
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2422
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Flow Cytometry
  • Immunoprecipitation (IP)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
❮❯

No additional charges, what you see is what you pay! *

CodeSizePrice
MBL-D161-3100 ug£301.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Show All

Further Information

Applications:
IP - 5 ug/250 mL of mouse bone marrow cells culture supernatant FCM - 5-10 ug/mL (final concentration)
Background:
?Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing α?β3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 4240 Mouse: 17304
Immunogen Translated:
Mouse peritoneal macrophages were attracted by Thioglycollate
Reactivity:
This antibody reacts with mouse MFG-E8 short and long form on Flow cytometry and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 2422) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell with Armenian hamster lymph node immunized with thioglycollate-elicited mouse peritoneal macrophages.
Target:
MFG-E8

Documents

References

1) Fens, M. H. A. M., et al., Blood 111, 4542-4550 (2008) 2) Montecalvo, A., et al., J. Immunol. 180, 3081-3090 (2008) 3) Hanayama, R., et al., Science 304, 1147-1150 (2004) 4) Morelli, A. E., et al., Blood 104, 3257-3266 (2004) 5) Hanayama, R., et al. J. Immunol. 172, 3876-3882 (2004) 6) Hanayama, R., et al. Nature 417, 182-187 (2002) Clone 2422 is used in these references.