Anti-GFP (Green Fluorescent Protein) mAb

Product Code:

MBL-D153-3

Product Group:

Primary Antibodies

Supplier:

MBL

Host Type:

Rat

Antibody Isotype:

IgG2a

Antibody Clonality:

Monoclonal

Antibody Clone:

RQ2

Regulatory Status:

RUO

Applications:

Immunocytochemistry (ICC)
Immunoprecipitation (IP)

Shipping:

4°C

Storage:

-20°C

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Code	Size	Price

MBL-D153-3

100 ul

£323.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Applications:

IP - 0.5-2 ug/sample ICC - 2 ug/mL

Background:

Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunoprecipitation.

Concentration:

1 mg/mL

Formulation:

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Immunogen Translated:

GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP

Reactivity:

This antibody reacts with GFP fusion protein on Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire

Shelf Life:

1 year

Source:

This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphonode immunized with GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP.

Target:

GFP

Documents

Datasheet

References

1) Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011) 2) Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009) 3) Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008) 4) Kato, A., et al., J. Virol. 82, 6172-6189 (2008) 5) Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006) 6) Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006) 7) Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006) 8) Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004)