MBL

Plk1 Positive Control

Product Code:
 
MBL-CY-E1163
Product Group:
 
Recombinant Proteins
Supplier:
 
MBL
Regulatory Status:
 
RUO
Shipping:
 
Dry Ice
Storage:
 
-70°C
 

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MBL-CY-E1163200 Assays£377.00
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This product comes from: United States.
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  • Further Information
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Further Information

Applications:
Other - 10 mUnits/well
Background:
Constitutive active human full length Plk1, containing one mutation, T210D in the active loop of the kinase domain. Also contains an N-terminal GST tag. Expressed in E. coli and purified by GSH agarose chromatography.
Formulation:
Supplied frozen in 20mM HEPES-KOH (pH 7.5), 1mMEDTA, 1 mM DTT, 50mM NaCl, 1% BSA, 0.03% Brij35and 50% glycerol.
Gene IDs:
Human: 5347 Mouse: 18817
MW:
Recombinant Plk1 demonstrates a single 92 kDa band by SDS-PAGE analysis.
Purity:
>85% pure as determined by SDS-PAGE analysis.
Shelf Life:
1 year
Target:
Plk1

Documents

References

1. Lane, H. A. & Nigg, E. A. Trends Cell Biol. 7, 63-68, 1997 2. Glover, D. M., et al. Genes Dev. 12, 3777-3787, 1998 3. Sunkel, C. E. & Glover, D. M. J. Cell Sci. 89, 25-38, 1988 4. Ohkura, H., et al. Genes Dev. 9, 1059-1073, 1995 5. Song, S. & Lee, K. S. J. Cell Biol. 152, 451-469, 2001 6. Lane, H. A. & Nigg, E. A. J. Cell Biol. 135, 1701-1713, 1996 7. Kotani, S., et al. Mol. Cell 1, 371-380, 1998 8. Alexandru, G., et al. Cell 105, 459-472, 2001 9. Liu, X.; Erikson, R. L. Proc. Nat. Acad. Sci. 100: 5789- 5794, 2003.