Human IL-18 ELISA Kit

interleukin 18 (interferon-γ-inducing factor)

Interleukin 18 (IL-18) is an 18 kDa cytokine which is identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock. It shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1β converting enzyme (ICE, caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It has been reported that IL-18 is produced from dendritic cells, activated macrophages, Kupffer cells, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalons. IFN-? is produced by activated T and NK cells and plays critical roles in the defense against microbial pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens expression, and inhibits osteoclast activation. IL-18 acts on T helper 1-type (Th1) cells, and in combination with IL-12 strongly induces production of IFN-γ by these cells. Pleiotropic effects of IL-18 have also been reported, including enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells. The ?Human IL-18 ELISA Kit? is a useful reagent for specifically measuring human IL-18 with high sensitivity by ELISA. This Kit does not detect 1 ng/mL of various cytokines, such as human IFN-α, IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-12, GM-CSF and murine IL-18. The results were all bellow the detection limit of 12.5 pg/mL.

The Human IL-18 ELISA kit measures human IL-18 by sandwich ELISA. The assay uses two monoclonal antibodies against two different epitopes of human IL- 18. In the wells coated with anti-human IL-18 monoclonal antibody, samples to be measured or standards are incubated. After washing, a peroxidase-conjugated anti-human IL-18 monoclonal antibody is added into the microwell and incubated. After another washing, the peroxidase substrate is mixed with the chromogen and allowed to incubate for an additional period of time. An acid solution is then added to each well to terminate the enzyme reaction and to stabilize the developed color. The optical density (O.D.) of each well is measured at 450 nm using a microplate reader. The concentration of human IL-18 is calibrated from a dose response curve based on reference standards.

Human: 3606 Mouse: 16173

Microwell strips coated with anti-Human IL-18 antibody 8-well strip x 12strips Human IL-18 calibrator (Lyophilized) 2 vials Conjugate reagent (Peroxidase conjugate anti-Human IL-18 monoclonal antibody) ( x 101) 0.2mL x 1vial Conjugate diluent (ready to use) 24mL x 1vial Assay diluent (ready to use) 30mL x 1vial Wash concentrate ( x 10) 100mL x 1vial Substrate reagent (TMB/H2O2) (ready to use) 20mL x 1vial Stop solution (2N H2SO4) (ready to use) (irritant) 18mL x ?vial

IL-18