Anti-CD117 (c-Kit) (Human) pAb

WB - 1:1000 (chemiluminescence detection system) IHC - 1:200 (Heat treatment required for paraffin) Microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5)

c-Kit, also known as stem cell factor receptor, steel factor receptor or CD117 is classified as a type III receptor tyrosine kinase (RTK) belonging to the platelet-derived growth factor receptor subfamily. Binding of stem cell factor (SCF), known as c-Kit ligand to c-Kit, initiates autophosphorylation of the receptor, subsequently leading to promotes a signal transduction cascade through Ras-Raf-MAP kinase cascade, phosphatidylinositol- 3-kinase, src family kinases, and JAK/STAT pathways. The roles of c-Kit include maturation of hematopoietic and primordial germ cells precursors and melanocytes during embryonic development. In acute myeloid leukemia (AML), c-Kit has been proposed to play a functional role, and becomes target molecule for drug development.

1 mg/mL in PBS/50% glycerol, pH 7.2

Human: 3815

Synthetic peptide C-terminal portion of c-kit gene product carrier protein binding (K963)

This antibody reacts with c-Kit (145 kDa) on Western blotting and Immunohistochemistry.

1 year

This antibody was purified from rabbit serum using the synthesized peptides (C-terminal of c-Kit gene product; K963) coupled protein A agarose column. The rabbit was immunized with carrier protein conjugated synthesized peptides (K963).

CD117