Anti-Mouse Siglec-F-BX016; Alexa Fluor™ 488 - RX016
Antibody Isotype:
IgG2a κ
Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Immunohistochemistry- Frozen Section (IHC-F)
- Spatial Biology
Storage:
This Phen°Cycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
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This product comes from:
US.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Siglec-F positive cells are expressed by eosinophils, alveolar macrophages, thymus and lung parenchyma tissues, and at very low levels on T cells. Siglec-F expression is restricted to cells of myelomonocytic lineage (CD11b-positive). Siglec-F is present on CD11blo (lo = low expressors) and CD11bhi (hi = low expressors) cell populations from mouse bone marrow, blood, and spleen but is especially high on the CD11blo cells. Cells that are CD11blo/Siglec-Fhi are predominantly immature cells of the myelomonocytic lineage, and cells that are CD11blo-hi/Siglec-Flo are mostly mature or immature neutrophils and monocytes, including leukocytes. Siglec-F is not expressed on mouse mast cells, stem cells, B cells, or NK cells.
Alexa Fluor™ 488, PhenoCycler®
This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Siglecs (sialic acid-binding immunoglobulin superfamily lectins) are a family of single pass, transmembrane cell surface proteins characterized by shared structural motifs and an ability to recognize sialic acids1,2. Siglec-F selectively recognizes 6'-sulfo-sialyl Lewis X as a glycan ligand, and this interaction is inhibited by the E50-2440 antibody3. Siglec-F belongs to the Siglec-3 (CD33) group4 and is a functionally convergent paralog of human Siglec-83. Mouse Siglec-F is used as a model to understand glycan based therapeutic targeting strategies in humans1, 2.
Expression of Siglec-F on eosinophils3 plays a role in the allergic inflammatory response, during which its surface levels on eosinophils and other cells increase1. Siglec-F deficient mice that are exposed to allergen sensitization and asthma-inducing conditions display more pronounced bone marrow, blood, and tissue eosinophilia due to reduced apoptosis1 as well as exaggerated eosinophilic inflammation and tissue remodeling in asthma models2. Administration of Siglec-F antibody reduces blood and tissue eosinophils in vivo2.
The rat monoclonal antibody E50-2440 was prepared by immunizing Lou rats with mSiglec-F-Fc, a fusion protein of the first two Ig-like domains of mouse Siglec-F and human IgG Fc fragment separated by an enterokinase cleavage site/FLAG tag (DYKDDDDK)4. Spleen cells were fused with Yb/20 myeloma cells, and hybridomas were screened by ELISA, tested by flow cytometry, and isotyped. The E50-2440 clone was selected based on its high staining intensity on primary cells. Siglec-F is a reliable marker of eosinophils among granulocytes in mouse1, and cellular staining profiles are identical in the C3H, Balb/c, and C57BL/6 mouse strains4.
Siglec-F