The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #:
KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Depending on the detection system that is used, colored precipitates, which can be clearly distinguished from the background, will be observed within the cells targeted by this Probe. A positive reactivity for human Alu repetitive sequences in the target cells is indicated by a distinctly stained nucleus. Visualization of signals should be performed by light microscopy using a 10x or 20x objective. For signal evaluation, necrotic, degenerated or over-digested cells should be avoided as these cells often stain nonspecifically. In order to judge the specificity of the hybridization signals and to confirm the correct performance of the method, any hybridization should be accompanied by controls. We recommend using at least one control sample containing both true positive and negative staining cells.
DNA (+) Control CISH Probe is designed to be used for the detection of human Alu repetitive sequences in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).
Bring probe to hybridization temperature before use.
