Ni-NTA Sepharose Purification Kit

Affinity purified Ni-NTA is conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins. The Ni-NTA sepharose is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells. The sepharose is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. The Ni-NTA resin can be used to purify 6 x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.