Krishgen Biosystems

KRIBIOLISA™ Protein A ELISA

Product Code:
 
KBBP11
Product Group:
 
ELISA Kits
Host Type:
 
Human
Regulatory Status:
 
RUO
Shipping:
 
2-8 ℃C
 

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KBBP111 x 96 wells£389.00
Quantity:
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This product comes from: India.
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Further Information

Calibration Range:
0 pg/ml - 4000 pg/ml
Detection Method:
Colorimetric, 450nm
Product Description:
Immunoassay for the measurement Natural & Structurally conserved Recombinant Protein A in samples containing Human immunoglobulin A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti- Protein A antibody coated on the microtitre plates. The wells are washed to remove any unbound reactants as per the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. . The wells are washed to remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. The substrate 3,3?,5,5? Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Protein A present.
Sample Type:
Cell Culture Supernatant and other Biological Preparations
Sensitivity:
Please refer to the current IFU for the updated calibrator range and sensitivity.