ProSci

GM-CSF Antibody

Product Code:
 
PSI-38-330
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect mGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
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To detect mGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.

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CodeSizePrice
PSI-38-330-0.05mg0.05mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-alpha
Application Note:
Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Western Blot
To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix.
NCBI Gene ID #:
12981
NCBI Official Name:
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol:
Csf2
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P01587
Protein GI Number:
P01587.1
Purification:
GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix
Research Area:
COVID-19,Cancer,Other,Immunology,Cell Cycle,Stem Cell
Swissprot #:
P01587
User NOte:
Centrifuge vial prior to opening.