Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
BCGF, BCDF, B cell stimulating factor (BSF-1)
Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-4.
Western Blot
To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues.
batch dependent
Unconjugated
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing immobilized Murine IL-4 matrix.
16189
interleukin 4
Il4
Mus musculus
Lyophilized
P07750
P07750.1
IL-4-specific antibody was purified by affinity chromatography employing immobilized Murine IL-4 matrix
COVID-19,Apoptosis,Cancer,Other,Cell Cycle,Stem Cell,Immunology
P07750
Centrifuge vial prior to opening.