ProSci

IL-7 Antibody (biotin)

Product Code:
 
PSI-38-317
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Human IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human IL-7.
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To detect Human IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human IL-7.

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CodeSizePrice
PSI-38-317-0.025mg0.025mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
Interleukin-7, Lymphopoietin 1(LP-1), pre-B cell factor
Application Note:
Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human IL-7.
Western Blot
To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-7 is a hematopoietic growth factor that primarily affects early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors, such as ConA and IL-2. Human and murine IL-7 are cross-species reactive. Recombinant Human IL-7 is a 17.5 kDa protein containing 153 amino acid residues.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-7. Anti-Human IL-7 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
3574
NCBI Official Name:
interleukin 7
NCBI Official Symbol:
IL7
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13232
Protein GI Number:
P13232.1
Purification:
IL-7 specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Cancer,Immunology,Stem Cell
Swissprot #:
P13232
User NOte:
Centrifuge vial prior to opening.