ProSci

G-CSF Antibody

Product Code:
 
PSI-38-310
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 ug/ml.
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In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 ug/ml.

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PSI-38-310-0.5mg0.5mg£476.00
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Further Information

Additional Names:
Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFbeta, pluripoietin
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci?s Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.
Western Blot
To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
Background:
G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography.
NCBI Gene ID #:
1440
NCBI Official Name:
colony stimulating factor 3
NCBI Official Symbol:
CSF3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P09919
Protein GI Number:
P09919.1
Purification:
G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography
Research Area:
Infectious Disease,Cancer,Obesity,Immunology,Stem Cell
Swissprot #:
P09919
User NOte:
Centrifuge vial prior to opening.