ELK Biotechnology

Human VC(Vitamin C) ELISA Kit

Product Code:
 
ELK9844
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9844-48T48T£261.00
Quantity:
ELK9844-96T96T£335.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
AA; L-Ascorbic Acid; Ascorbate
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
625-40000 ng/mL
Research Area:
Metabolic pathway;Infection immunity;Nutrition metabolism;
Sample type:
Serum, plasma and other biological fluids
Sensitivity:
192.1 ng/mL
Standard:
40000 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin C(VC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin C(VC). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin C(VC) in the samples is then determined by comparing the OD of the samples to the standard curve.