ELK Biotechnology

General F-E3(Free Estriol) ELISA Kit

Product Code:
 
ELK9646
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9646-48T48T£261.00
Quantity:
ELK9646-96T96T£335.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
F-E3; Free Estriol
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
0.63-40 ng/mL
Research Area:
Endocrinology;Reproductive science;Hormone metabolism;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.218 ng/mL
Standard:
40 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Estriol(F-E3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Estriol(F-E3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Estriol(F-E3) in the samples is then determined by comparing the OD of the samples to the standard curve.