ELK Biotechnology

General MEK(Methionine-Enkephalin) ELISA Kit

Product Code:
 
ELK9576
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9576-48T48T£298.00
Quantity:
ELK9576-96T96T£390.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
Met-enkephalin; Opioid growth factor; OGF
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
125.00-8000 pg/mL
Research Area:
Signal Transduction
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
75.00 pg/mL
Standard:
8000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Methionine-Enkephalin(MEK) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Methionine-Enkephalin(MEK). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine-Enkephalin(MEK) in the samples is then determined by comparing the OD of the samples to the standard curve.