Human PTPN5(Tyrosine-Protein Phosphatase Non-Receptor Type 5) ELISA Kit

FLJ14427; Neural specific protein tyrosine phosphatase; Neural-specific protein-tyrosine phosphatase; Protein tyrosine phosphatase non receptor type 5 (striatum enriched); Protein tyrosine phosphatase non receptor type 5; Protein tyrosine phosphatase striatum enriched; PTN5; PTN5_HUMAN; PTP STEP; PTPN 5; Ptpn5; PTPSTEP; STEP; Striatum-enriched protein-tyrosine phosphatase; Tyrosine protein phosphatase non receptor type 5; Tyrosine-protein phosphatase non-receptor type 5;

3.5h

Sandwich

0.16-10 ng/mL

Neuro science; Signal Transduction

tissue homogenates, cell lysates and other biological fluids

0.054 ng/mL

10 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5) in the samples is then determined by comparing the OD of the samples to the standard curve.

P54829