ELK Biotechnology

General 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Product Code:
 
ELK9387
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9387-48T48T£261.00
Quantity:
ELK9387-96T96T£335.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
25 Hydroxy Vitamin D;
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
3.13-200 ng/mL
Research Area:
Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
0.91 ng/mL
Standard:
200 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25 Hydroxy Vitamin D(25-OH-D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25 Hydroxy Vitamin D(25-OH-D). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25 Hydroxy Vitamin D(25-OH-D) in the samples is then determined by comparing the OD of the samples to the standard curve.