ProSci

PD-1 Antibody [J43], Rabbit IgG

Product Code:
 
PSI-24-712
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
J43
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Depletion
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Neutralisation
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow cytometry using the Anti-PD-1 antibody J43 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of J43 (24-712- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using Anti-PD-1 antibody J43 ( </b> Mouse lymph node tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of J43 (24-712) at 0.003ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Binding curve of anti-PD-1 antibody J43 (24-712) to recombinant mouse PD-1 Fc-Fusion Protein. </b> ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein (Pr00152-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using 24-712. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.
❮❯

<b> Flow cytometry using the Anti-PD-1 antibody J43 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of J43 (24-712- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using Anti-PD-1 antibody J43 ( </b> Mouse lymph node tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of J43 (24-712) at 0.003ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Binding curve of anti-PD-1 antibody J43 (24-712) to recombinant mouse PD-1 Fc-Fusion Protein. </b> ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein (Pr00152-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using 24-712. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.

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CodeSizePrice
PSI-24-712-0.2mg0.2mg£681.00
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Further Information

Additional Names:
CD279, PD1, PD 1, Programmed cell death protein 1, Programmed cell death 1, pdcd1, Protein PD-1, mPD-1
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
NCBI Gene ID #:
18566
NCBI Official Name:
programmed cell death 1
NCBI Official Symbol:
Pdcd1
NCBI Organism:
Mus musculus
Research Area:
Apoptosis, Immunology
Swissprot #:
Q02242
User NOte:
For research use only.