ProSci

IL-17F Antibody [8F5.1A9], Rabbit IgG

Product Code:
 
PSI-24-678
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8F5.1A9
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Flow Cytometry
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 8F5.1A9 (24-678- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 8F5.1A9 (24-678- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

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PSI-24-678-0.2mg0.2mg£681.00
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Further Information

Additional Names:
Interleukin-17F, IL17F
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
NCBI Gene ID #:
257630
NCBI Official Name:
interleukin 17F
NCBI Official Symbol:
Il17f
NCBI Organism:
Mus musculus
Research Area:
Chemokines & Cytokines
Swissprot #:
Q7TNI7
User NOte:
For research use only.