NSJ Bioreagents

SCC112 Antibody / PSD5A

Product Code:
 
NSJ-RQ6505
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PSD5A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PSD5A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human HEK293, 3) rat stomach, 4) rat pancreas, 5) mouse stomach and 6) mouse pancreas lysate with PSD5A antibody. Predicted molecular weight ~151 kDa.
3 / 3
Flow cytometry testing of human A431 cells with PSD5A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSD5A antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PSD5A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) rat stomach, 4) rat pancreas, 5) mouse stomach and 6) mouse pancreas lysate with PSD5A antibody. Predicted molecular weight ~151 kDa.
Flow cytometry testing of human A431 cells with PSD5A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSD5A antibody.

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CodeSizePrice
NSJ-RQ6505-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PSD5A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Sister chromatid cohesion protein PDS5 homolog A is a protein that in humans is encoded by the PDS5A gene. The protein encoded by this gene binds to the cohesin complex and associates with chromatin through most of the cell cycle. The encoded protein may play a role in regulating sister chromatid cohesion during mitosis. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids M1-R1337) was used as the immunogen for the PSD5A antibody.
Limitation:
This PSD5A antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q29RF7