NSJ Bioreagents

MGEA5 Antibody / OGA

Product Code:
 
NSJ-RQ6503
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the O-GlcNAcase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human T-47D cells with O-GlcNAcase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human rat spleen tissue with O-GlcNAcase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain and 4) mouse brain lysate with O-GlcNAcase antibody. Predicted molecular weight ~103 kDa, routinely observed at ~130 kDa (cytoplasmic form) and ~75 kDa (nuclear form).
4 / 4
Flow cytometry testing of human U-87 MG cells with O-GlcNAcase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= O-GlcNAcase antibody.
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Immunofluorescent staining of FFPE human T-47D cells with O-GlcNAcase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rat spleen tissue with O-GlcNAcase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain and 4) mouse brain lysate with O-GlcNAcase antibody. Predicted molecular weight ~103 kDa, routinely observed at ~130 kDa (cytoplasmic form) and ~75 kDa (nuclear form).
Flow cytometry testing of human U-87 MG cells with O-GlcNAcase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= O-GlcNAcase antibody.

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CodeSizePrice
NSJ-RQ6503-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the O-GlcNAcase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein O-GlcNAcase (OGA) is an enzyme with systematic name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KVYTIRPYFPKDEASVYKICRE from the human protein were used as the immunogen for the O-GlcNAcase antibody.
Limitation:
This O-GlcNAcase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O60502