NSJ Bioreagents

Polycystin 2 Antibody

Product Code:
 
NSJ-RQ5595
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Polycystin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human renal cancer with Polycystin 2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) human U-87 MG, 2) rat spleen, 3) mouse thymus, 4) mouse lung and 5) mouse SP2/0 lysate with Polycystin 2 antibody. Predicted molecular weight: ~110 kDa.
3 / 3
Flow cytometry testing of human A549 cells with Polycystin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Polycystin 2 antibody.
❮❯

IHC staining of FFPE human renal cancer with Polycystin 2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) rat spleen, 3) mouse thymus, 4) mouse lung and 5) mouse SP2/0 lysate with Polycystin 2 antibody. Predicted molecular weight: ~110 kDa.
Flow cytometry testing of human A549 cells with Polycystin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Polycystin 2 antibody.

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CodeSizePrice
NSJ-RQ5595-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Polycystin 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Polycystin-2 is a protein that in humans is encoded by the PKD2 gene. It is mapped to 4q22.1. This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids S829-R893) was used as the immunogen for the Polycystin 2 antibody.
Limitation:
This Polycystin 2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13563