NSJ Bioreagents

ALDH1A1 Antibody / Acetaldehyde dehydrogenase 1

Product Code:
 
NSJ-RQ5510
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ALDH1A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 10
Immunofluorescent staining of FFPE human A549 with ALDH1A1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 10
Immunofluorescent staining of FFPE human A431 with ALDH1A1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 10
Flow cytometry testing of human HepG2 cells with ALDH1A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A1 antibody.
4 / 10
Flow cytometry testing of human HL60 cells with ALDH1A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A1 antibody.
5 / 10
IHC staining of FFPE human breast cancer with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human renal cancer with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE mouse stomach with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE rat stomach with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 10
Western blot testing of human 1) placenta, 2) A549 and 3) HepG2 lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.
10 / 10
Western blot testing of 1) rat liver, 2) rat lung, 3) rat kidney, 4) mouse liver, 5) mouse lung and 6) mouse kidney lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.
❮❯

Immunofluorescent staining of FFPE human A549 with ALDH1A1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 with ALDH1A1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human HepG2 cells with ALDH1A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A1 antibody.
Flow cytometry testing of human HL60 cells with ALDH1A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A1 antibody.
IHC staining of FFPE human breast cancer with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse stomach with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat stomach with ALDH1A1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) A549 and 3) HepG2 lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.
Western blot testing of 1) rat liver, 2) rat lung, 3) rat kidney, 4) mouse liver, 5) mouse lung and 6) mouse kidney lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.

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CodeSizePrice
NSJ-RQ5510-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ALDH1A1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that in humans is encoded by the ALDH1A1 gene. It is mapped to 9q21.13. The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids T6-Q342) was used as the immunogen for the ALDH1A1 antibody.
Limitation:
This ALDH1A1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00352