NSJ Bioreagents

PCNA Antibody

Product Code:
 
NSJ-RQ5380
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DO-16
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the PCNA antibody at -20oC.
1 / 2
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
2 / 2
IHC staining of FFPE human colon tissue with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
IHC staining of FFPE human colon tissue with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ5380-100ul100 ul£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:3000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the PCNA antibody should be determined by the researcher.
Description:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human PCNA was used as the immunogen for the PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P12004