NSJ Bioreagents

CD63 Antibody / LAMP-3

Product Code:
 
NSJ-V8875
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LAMP3/4949
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).
❮❯

IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V8875-20UG20ug£264.00
Quantity:
NSJ-V8875-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
ELISA: 1-5ug/ml (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CD63 antibody should be determined by the researcher.
Description:
CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Full-length human CD63 protein was used as the immunogen for the CD63 antibody.
Limitation:
This CD63 antibody is available for research use only.
Localization:
Cell surface, Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P08962