NSJ Bioreagents

Biotin / Vitamin B7 / Vitamin H Antibody

Product Code:
 
NSJ-V8868
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BTN399
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BS
❮❯

IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BS

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CodeSizePrice
NSJ-V8868-20UG20ug£264.00
Quantity:
NSJ-V8868-100UG100ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the Biotin antibody should be determined by the researcher.
Description:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Limitation:
This Biotin antibody is available for research use only.
Purity:
Protein A/G affinity
Species Reactivity :
All species
Uniprot #:
Not Applicable