NSJ Bioreagents

CA8 Antibody / Carbonic Anhydrase VIII

Product Code:
 
NSJ-V8822
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CA8/6572
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Carbonic Anhydrase VIII antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human brain tissue with Carbonic Anhydrase VIII antibody (clone CA8/6572). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
SDS-PAGE analysis of purified, BSA-free Carbonic Anhydrase VIII antibody (CA8/6572) as confirmation of integrity and purity.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Carbonic Anhydrase VIII antibody (clone CA8/6572). These results demonstrate the foremost specificity of the CA8/6572 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human brain tissue with Carbonic Anhydrase VIII antibody (clone CA8/6572). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Carbonic Anhydrase VIII antibody (CA8/6572) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Carbonic Anhydrase VIII antibody (clone CA8/6572). These results demonstrate the foremost specificity of the CA8/6572 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V8822-20UG20ug£264.00
Quantity:
NSJ-V8822-100UG100ug£534.00
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Carbonic Anhydrase VIII antibody should be determined by the researcher.
Description:
Carbonic anhydrase-related protein is an protein that in humans is encoded by the CA8 gene. The CA8 protein lacks the catalytic activity of other carbonic anhydrase enzymes. A rare, autosomal recessive form of cerebellar ataxia known as cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CAMRQ3) is caused by mutations in the CA8 gene. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Carbonic Anhydrase VIII/CA8 protein was used as the immunogen for the Carbonic Anhydrase VIII antibody.
Limitation:
This Carbonic Anhydrase VIII antibody is available for research use only.
Localization:
Cytoplasm, Cell surface
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P35219