NSJ Bioreagents

CFTR Antibody / Cystic Fibrosis Transmembrane Regulator

Product Code:
 
NSJ-V9382
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CFTR/7003R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant CFTR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human kidney tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human pancreatic tissue with recombinant CFTR antibody (clone CFTR/7003R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human ovarian carcinoma tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant CFTR antibody (clone CFTR/7003R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human kidney tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with recombinant CFTR antibody (clone CFTR/7003R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant CFTR antibody (clone CFTR/7003R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CFTR antibody (clone CFTR/7003R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9382-20UG20ug£264.00
Quantity:
NSJ-V9382-100UG100ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant CFTR antibody should be determined by the researcher.
Description:
Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 550-850 was used as the immunogen for the recombinant CFTR antibody.
Limitation:
This recombinant CFTR antibody is available for research use only.
Localization:
Cell surface, Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P13569