Histone H3 Antibody (pS10)

Immunohistochemistry (FFPE): 1-2ug/ml

Optimal dilution of the phospho-Histone H3 antibody should be determined by the researcher.

Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Amino acids ARK(pS)TGGKAPRKQLc) was used as the immunogen for the phospho-Histone H3 antibody.

This phospho-Histone H3 antibody is available for research use only.

Nucleus, Chromosome

Protein A/G affinity

Human

P68431