NSJ Bioreagents

HSP27 Antibody / HSPB1

Product Code:
 
NSJ-V8989
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rHSPB1/6489
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant HSPB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human liver tissue lysate using recombinant HSPB1 antibody (clone rHSPB1/6489). Predicted molecular weight: 23-27 kDa.
2 / 3
IHC staining of FFPE human thyroid with recombinant HSPB1 antibody (clone rHSPB1/6489). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant HSPB1 antibody (clone rHSPB1/6489) as confirmation of integrity and purity.
❮❯

Western blot testing of human liver tissue lysate using recombinant HSPB1 antibody (clone rHSPB1/6489). Predicted molecular weight: 23-27 kDa.
IHC staining of FFPE human thyroid with recombinant HSPB1 antibody (clone rHSPB1/6489). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSPB1 antibody (clone rHSPB1/6489) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8989-20UG20ug£264.00
Quantity:
NSJ-V8989-100UG100ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant HSPB1 antibody should be determined by the researcher.
Description:
HSP27 is a small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length HSP27 protein was used as the immunogen for the recombinant HSPB1 antibody.
Limitation:
This recombinant HSPB1 antibody is available for research use only.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P04792