NSJ Bioreagents

Topoisomerase II alpha Antibody / TOP2A

Product Code:
 
NSJ-F55028
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human skin tissue with Topoisomerase II alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with Topoisomerase II alpha antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.
4 / 4
Western blot testing of human A375 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.
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IHC testing of FFPE human skin tissue with Topoisomerase II alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Topoisomerase II alpha antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.
Western blot testing of human A375 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.

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CodeSizePrice
NSJ-F55028-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55028-0.4ML0.4 ml£502.00
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Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1167-1196 from the human protein was used as the immunogen for the Topoisomerase II alpha antibody.
Limitation:
This Topoisomerase II alpha antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P11388