NSJ Bioreagents

EFEMP1 Antibody

Product Code:
 
NSJ-F55016
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the EFEMP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) A431 and 2) HUVEC lysate with EFEMP1 antibody. Predicted molecular weight ~54 kDa.
2 / 4
Western blot testing of mouse lung tissue lysate with EFEMP1 antibody. Predicted molecular weight ~54 kDa.
3 / 4
IHC testing of FFPE human lung carcinoma tissue with EFEMP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human NCI-H460 cells with EFEMP1 antibody; Blue=isotype control, Green= EFEMP1 antibody.

Western blot testing of human 1) A431 and 2) HUVEC lysate with EFEMP1 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of mouse lung tissue lysate with EFEMP1 antibody. Predicted molecular weight ~54 kDa.
IHC testing of FFPE human lung carcinoma tissue with EFEMP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H460 cells with EFEMP1 antibody; Blue=isotype control, Green= EFEMP1 antibody.

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CodeSizePrice
NSJ-F55016-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55016-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
EFEMP1 encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, EGF-containing fibulin-like extracellular matrix protein 1 contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 119-148 from the human protein was used as the immunogen for the EFEMP1 antibody.
Limitation:
This EFEMP1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q12805