ELK Biotechnology

Human LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit

Product Code:
 
ELK1606
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK1606-48T48T£298.00
Quantity:
ELK1606-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.274 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.