NSJ Bioreagents

Cd20 Antibody

Product Code:
 
NSJ-RQ6329
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Cd20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse spleen tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE mouse intestinal tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of mouse spleen tissue lysate with Cd20 antibody. Expected molecular weight ~33 kDa but may be observed at up to ~50 kDa due to glycosylation.
4 / 4
Flow cytometry testing of mouse HEPA1-6 cells with Cd20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd20 antibody.
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IHC staining of FFPE mouse spleen tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestinal tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse spleen tissue lysate with Cd20 antibody. Expected molecular weight ~33 kDa but may be observed at up to ~50 kDa due to glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with Cd20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd20 antibody.

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CodeSizePrice
NSJ-RQ6329-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd20 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What�s more, this protein may be involved in the regulation of B-cell activation and proliferation.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids M1-P291) was used as the immunogen for the Cd20 antibody.
Limitation:
This Cd20 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Mouse
Uniprot #:
P11836