NSJ Bioreagents

MEKK2 Antibody

Product Code:
 
NSJ-RQ6104
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MEKK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human A431 cells with MEKK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562, 4) HepG2, 5) MCF7, 6) Jurkat and 7) rat kidney lysate with MEKK2 antibody. Predicted molecular weight ~78 kDa.
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Immunofluorescent staining of FFPE human A431 cells with MEKK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562, 4) HepG2, 5) MCF7, 6) Jurkat and 7) rat kidney lysate with MEKK2 antibody. Predicted molecular weight ~78 kDa.

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CodeSizePrice
NSJ-RQ6104-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MEKK2 antibody should be determined by the researcher.
Description:
MAP3K2, mitogen-activated protein kinase kinase kinase 2, also known as MEKK2, belongs to the mitogen-activated protein kinase (MAPK) kinase kinase gene family, which is involved in regulating multiple MAPK signaling pathways. Endogenous MAP3K2 localized mainly to the cytosol of unstimulated HeLa cells and rat fibroblasts. The MAP3K2 gene is mapped to chromosome 2 by the International Radiation Hybrid Mapping Consortium. MAP3K2 is a major upstream activator of the JNK, MAPK cascade. MAP3K2 was identifies as 1 of 6 kinases that regulate different steps of the caveolar cycle. Silencing of MAP3K2 strongly reduced kiss-and-run dynamics, leading to an accumulation of caveolar structures at the cell surface without affecting caveolae coat assembly or clustering.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-Q364) was used as the immunogen for the MEKK2 antibody.
Limitation:
This MEKK2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
Q9Y2U5