NSJ Bioreagents

CD15 Antibody / SSEA-1

Product Code:
 
NSJ-V3203
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
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IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

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CodeSizePrice
NSJ-V3203-20UG20 ug£264.00
Quantity:
NSJ-V3203-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 5-10ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD15 antibody should be determined by the researcher.
Description:
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Purified human neutrophils were used as the immunogen for the CD15 antibody.
Limitation:
This CD15 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein A affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P22083