NSJ Bioreagents

Map4k3 Antibody / Mekkk3 / Glk

Product Code:
 
NSJ-F54692
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Mekkk3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of mouse Neuro-2a cell lysate with Mekkk3 antibody. Predicted molecular weight ~101 kDa.
2 / 4
Western blot testing of human HepG2 cell lysate with Mekkk3 antibody. Predicted molecular weight ~101 kDa.
3 / 4
IHC testing of FFPE mouse heart tissue with Mekkk3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with Mekkk3 antibody; left histogram=isotype control, right histogram= Mekkk3 antibody.
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Western blot testing of mouse Neuro-2a cell lysate with Mekkk3 antibody. Predicted molecular weight ~101 kDa.
Western blot testing of human HepG2 cell lysate with Mekkk3 antibody. Predicted molecular weight ~101 kDa.
IHC testing of FFPE mouse heart tissue with Mekkk3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of mouse Neuro-2a cells with Mekkk3 antibody; left histogram=isotype control, right histogram= Mekkk3 antibody.

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CodeSizePrice
NSJ-F54692-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54692-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Mekkk3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MEKKK3 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 501-529 from the mouse protein was used as the immunogen for the Mekkk3 antibody.
Limitation:
This Mekkk3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Human
Uniprot #:
Q99JP0